Pierre Taberlet, Centre National de la Recherche Scientifique “Laboratoire d’Ecologie Alpine”. Pierre Taberlet studied biology and geology at the University Joseph Fourier of

Grenoble (France) from 1972 to 1976. He was a biology teacher in a high school from 1978 to 1989. He obtained a PhD in 1992 from the University Joseph Fourier, and the

Habilitation in 1993. He joined the Centre National de la Recherche Scientifique in 1994.He has worked in the “Laboratoire d’Ecologie Alpine” (Grenoble), first as junior scientist (1994-1998), and then as senior scientist (1999-2019, and then Emeritus). He was the head of this laboratory from 1999 to 2010. For the past 20 years, his research team has been working in the field of molecular ecology, with emphasis on the phylogeography of wild animals and plants, on conservation genetics (development of non-invasive

sampling), and on domestication of goats and sheep. More recently, he is involved in biodiversity assessment using environmental DNA (from soil, water, and feces). He is author or co-author of more than 300 scientific publications in peer-reviewed journals and is highly cited researcher 2022 (https://clarivate.com/highly-cited-researchers/) in the category "Environment and Ecology" (highly cited in this category since 2014). He iscurrently senior editor of Molecular Ecology and Molecular Ecology Resources, associate

editor of Science Advances, and Section Editor for Science China Life Sciences. He received the Molecular Ecology Prize in 2007, and coordinated three large collaborative

European projects between 2004 and 2014.

Environmental DNA is not magic

The talk will begin with a brief introduction to environmental DNA, including a few definitions and the historical background to its emergence. As with any technological and

methodological innovation in science, there is a risk that false results will be published, even in the best international journals. This risk is illustrated by a few examples of publications in the field of ancient DNA. The same risk exists for environmental DNA. I will first analyze in detail an article published in 2016 whose results were not subsequently replicated (Deiner etal. 2016, Nature Communications, 7, 12544), then review several articles on airborne DNA.Finally, the talk will list best practices for securing results obtained with environmental DNA.

姚蒙，北京大学生态研究中心副研究员。北京大学生物化学及分子生物学学士，密西根大学(University of Michigan)分子细胞及发育生物学博士，密西根大学医学院博士后，2010年至今于北京大学工作。研究领域为分子生态学及保护遗传学，近年来研究课题聚焦于应用环境DNA的生物多样性分析，基于分子条形码的食肉动物食物网关系，以及多种受威胁物种的种群遗传学。迄今发表SCI论文近40篇，近5年作为通讯作者发表于Science Advances，Current Biology，Frontiers in Ecology and the Environment，Environmental Science & Technology，Methods in Ecology and Evolution等期刊。现任Environmental DNA国际期刊副主编。主持和参加多项科研项目，包括国家自然科学基金面上项目、国家重点研发计划、第二次青藏高原综合科学考察项目等。

eDNA揭示青藏高原水生态系统多类群生物多样性

青藏高原蕴藏着丰富的淡水资源，是亚洲重要的水源地，同时也是全球气候变化下最为敏感和脆弱的地区之一。了解青藏高原水生生物多样性组成和分布特征对预测和应对气候变化对高原水生态系统服务功能的影响具有重要意义。然而高原严酷的自然环境对系统调查生物多样性造成极大困难。新兴的环境DNA（eDNA）方法利用生物释放到环境中的DNA检测物种分布和群落构成，可以高效且无损伤性地进行生物多样性研究。我们利用eDNA结合多分子标记宏条形码（multi-marker metabarcoding）方法，针对青藏高原典型河流、湖泊等进行生物多样性分析，系统探究从原核到真核、从藻类到动物、从水生到陆生生物等生态系统各主要类群的多样性分布模式和群落结构特征，并结合环境因子分析影响不同类群群落组成的因素及群落构建机制。eDNA方法同时对多类群生物多样性格局的解析有助于增进对高原生态系统结构和功能的全面了解，并为准确及时地捕获群落动态提供有力支撑。